Mother species → original species
When buying a test tube mother, it is necessary to pay attention to the superiority and inferiority of the mother seed, and carefully check the growth of the mycelium in the test tube so as to avoid buying an aging mother seed. In general, a robust mother breed is characterized by a white, thick, and neat tip. If the mycelium is dry or the medium shrinks, there is yellow water at the bottom of the bottle, indicating that the hypha is aging. Please do not purchase it.
From the parent species to the original species, it is the first time that strains have expanded their culture. specific method:
Material preparation: test tube masterbatch, beer bottle (preparation of 12 beer bottles inoculated according to one test tube masterbatch); preparation of corn kernels or wheat granules (according to a beer bottle containing 250-300 g corn kernels or wheat granules )Wait.
Operation process: first deal with culture materials, if you choose to use wheat, you need to soak it in water for 24 hours (in the middle for 3 times of water, to prevent acidification); if you use corn kernels, because of its thick seed coat, not easy to bubble through, need to Use it after it is cooked. Note that when cooking corn kernels, it is necessary to grasp the heat, neither to allow corn grains to “flower” nor to ripen endogenously.
When the amount of culture material in the beer bottle is 4/5 of the bottle, it is sealed with a tampon and wrapped with kraft paper, so as to prevent the steam from soaking the tampon during high temperature sterilization. The bottle was then placed on a steamer and autoclaved for about 12 hours.
After sterilization, the bottle temperature is reduced to 30 °C, and then transferred to the sterile inoculation box for inoculation, that is, using a vaccination needle to pick up a little hyphae from the mother tube, quickly into the beer bottle, with cotton Sealed and wrapped in kraft paper. Then, inoculated beer bottles were stored in a room at a temperature of about 25°C to promote mycelial growth. Usually after 20 to 25 days the original species can be obtained.
Reminder: The good original species of mycelium is dense, white, uniform, thick, cotton wool-like, and there are wall climbing phenomenon. After the original species grows over the bottle, it needs to be expanded and cultivated as a cultivar immediately. Otherwise, once the nutrients are exhausted, the mycelium will grow old and even die.
Original species → First-class cultivars
The original species is expanded by cultivating. However, due to the small number of original species, if the mushroom is directly transferred to the mushroom bag for mushroom production, it will not only result in prolonged bacterial growth due to less strains and weaker bacteria, but also cause mushrooming due to inconsistent growth rates of both ends of the bag. Uneven. Therefore, it is recommended that the mushroom farmer adopt the seed production mode after the first pouch, that is, inoculate the original seed into a small bacterial bag for seed production in a small bag, and then transfer it to a large bacterial bag for formal cultivation. This ensures that Sufficient germination rate and uniformity, and strong bacteria potential, the chance of infection with bacteria is small.
Material Preparation: Level 1 cotton shell (wet with water until fingertips have water without dropping drops, add appropriate amount of lime to adjust pH, stir evenly); small bacteria bags (specification 18cm 40cm 0.03cm Or 22 cm 45 cm 0.04 cm polyethylene plastic bags, the amount of 10 micro-bacterial bags in accordance with a beer bottle to prepare) and so on.
The operation process is as follows: Firstly, the pre-prepared culture cotton seed shell is placed in a small bacteria bag, the two ends are fastened, and then placed in a steamer to be sterilized at a high temperature, and when the temperature reaches 100° C. for 20 hours. After sterilization, cool it in a cool and ventilated place and inoculate it when the temperature is below 30°C. In order to facilitate the inoculation, you can use the inoculation spoon to pour all the original species in the beer bottle in the small basin, or use tape to wrap the beer bottle for a few times, then break the bottle, remove the broken glass, and put the original species into the small basin. Stay in use. When inoculating, use the inoculation spoon to insert the original species into both ends of the microflora. Finally, the small bacterium bags should be placed in a place where the temperature is relatively constant. Usually about 20 days, the hyphae can grow over the whole bag.
Reminder: Pay attention to the small bacterium bags when you place them, because there is still residual heat in the bacterium bags, and the hyphae will also generate a lot of heat during growth. Therefore, it is not suitable to stack too high, generally "well" shaped to stack 3 to 4 layers, in order to facilitate ventilation and heat, to avoid "burn bacteria." In addition, the first-level cultivars must often be bagged during the cultivation process to prevent heat accumulation. At the same time, the growth of the mycelia and the presence or absence of infection should be checked. Generally, the entire culture process can be bagged three times. After the mycelium is full, it needs to be transferred to the big bacteria bag immediately, otherwise it may cause the aging of the mycelia and reduce its production efficiency.
Primary cultivar → Secondary cultivar
At present, the cultivation of Pleurotus ostreatus is mainly based on raw material cultivation, and the culture materials are mainly cotton husks, and the appropriate amount of corn core, bran, lime or gypsum, sugar or corn flour, trace elements, and inorganic salts are added in order to provide comprehensive nutrition. balanced. Since raw materials are cultivated, the culture materials must be sterilized before use. Method: When the culture material is mixed with water, mix clotrimidine (sodium dichloroisocyanurate), mix 500 kg of culture material per 0.5 kg of clotrinone, and then cover it with a plastic film, stuffy for about 12 hours, and finally The culture material can be divided into large bacteria bags. Note that this link should be used in the "four-layer bacteria three-layer material" method of sub-planting, in order to speed up the speed of bacteria, improve the effect of fruiting. According to this method, a small bag of cultivars can be divided into 2 to 3 large bags.
